General description

A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K_D = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC₅₀ = ~ 3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566661) in H₂O is also available.

A cell-permeable Smoothened Agonist, SAG, CAS 364590-63-6, modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K_D = 59 nM).

A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K_D = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC₅₀ ~3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA.107, 9323.Meloni, A.R., et al. 2006. Mol. Cell. Biol.26, 7750.Chen, W., et al. 2004. Science306, 2257.Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.

Packaging

1, 5 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)